The CCS will grow mammalian or insect cells for monoclonal antibody production, recombinant protein production or to harvest large numbers of cells. To this end there are several options available:
- Roller bottle culture
- The CCSF can grow suspension or adherent cells in roller bottles and produce up to 10L of antibody/recombinant protein containing supernatant. Or we can provide the researcher with the cell pellet.
- CelLine Flask culture
- Adherent or suspension cells can be grown in the CelLine flask for long periods of time.
- Cells are grown at high density and concentrated supernatant is obtained at regular intervals, which can be given to the customer or CCSF can purify the protein from the supernatant.
- Hollow-fiber bioreactor
- This method of growing cells is best when multi-milligram amounts of antibody or recombinant protein need to be produced. The hollow-fiber cartridge is inoculated with cells and cells are grown on a continuous basis. 10 – 50ml of highly concentrated supernatant are harvested every day (except on weekends) and frozen immediately. Proteins produced in the hollow-fiber bioreactor are typically more homogeneous and generally of higher quality than proteins produced in other systems. A hollow-fiber cartridge can be kept going for as long as desired.
- Purification of antibody or tagged recombinant proteins
- IgG is purified by Protein G chromatography on FPLC.
- His-tagged proteins are purified with NiNTA.
- We may be able to purify other tagged proteins.
Please contact Sabine Baxter for details.
