Cell Culture Training Schedule
The facility trains laboratory personnel in basic sterile and tissue culture techniques.
Day 1
- Discuss the basics about mammalian cell culture and tissue culture laboratory set up including culture hood, CO 2 incubator, water bath and inverted microscope. Explain media preparation and the use of two cell lines- SP2/O (suspension) and NIH3T3 (attached).
- Show how to prepare media.
- Start the culture of one cell line SP2/O or NIH3T3.
Day 3
- Look at the cells under an inverted microscope, explain cell viability.
- Counting of cells by hemocytometer.
- Depending on the cell count, proceed to split the cells.
- The trainee can prepare the media for the other cell line.
Day 5
- Start the other line, explain the properties of attached cells vs the cells in suspension, how to harvest cells.
- Continue working with the first cell line, make flask for the weekend.
Day 8
- The cells (Ist line) are ready to freeze, explain cell freezing procedure, and freeze two-three vials.
- Trypsinize and split the attached cells, count cells.
Day 10
- Freeze the attached cells.
Day 12
- Start one of the frozen vials. Discussion of cell culture procedures with Dr. T. Ganguly.