Oligonucleotide Synthesis
The Stockroom offers oligonucleotide synthesis through three different vendors:
**All orders that have 10 or more sequences must be submitted in excel format. Four columns are required; Sequence name, Sequence, Scale, Purification.**
IDT (Integrated DNA Technologies)
Click here to place an order.
Pricing Information
| Description | Unit | Price |
|---|---|---|
| Base Charges: | ||
| Oligo 25 nmole | per base | $ 0.24 |
| Oligo 100 nmole | per base | $ 0.41 |
| Oligo 250 nmole | per base | $ 0.63 |
| Oligo 1000 nmole | per base | $ 1.15 |
| Oligo - 10 umole scale | per base | $ 16.00 |
| Chimeric DNA base | per base | $ 2.48 |
| RNA base, 5 OD scale | per base | $ 3.47 |
| Phosphorothioate, 100/250nmole | per base | $ 3.33 |
| Phosphorothioate, 1000nmole | per base | $ 4.76 |
| Set-Up Charges: | ||
| Dual Labeled 5' TET/ 3' BHQ, HPLC, 100nm | ea | $ 189.00 |
| Dual Labeled 6FAM/BHQ, HPLC, 100 nmole | ea | $ 175.00 |
| Dual Labeled 6FAM/TAMARA, 100 nmole | ea | $ 175.00 |
| Dual labeled Probe - 6FAM/TAMARA, 250 nmole | ea | $ 250.00 |
| Dual PAGE & RP-HPLC | ea | $ 85.00 |
| Dual PAGE & RP-HPLC | ea | $ 125.00 |
| Dual PAGE & RP-HPLC | ea | $ 230.00 |
| HPLC-100 nmole set up | ea | $ 40.00 |
| HPLC-1000 nmole set up | ea | $ 95.00 |
| HPLC-250 nmole set up | ea | $ 60.00 |
| IEHPLC-100 nmole set up | ea | $ 40.00 |
| IE-HPLC-1000 nmole set up | ea | $ 95.00 |
| IE-HPLC-250 nmole set up | ea | $ 60.00 |
| PAGE-100 nmole set up | ea | $ 40.00 |
| PAGE-1000 nmole set up | ea | $ 95.00 |
| PAGE-250 nmole set up | ea | $ 60.00 |
| Set Up - RNase Free HPLC, 100 nmole | ea | $ 65.00 |
| Set Up - RNase Free HPLC, 250 nmole | ea | $ 91.50 |
| Dyes and Other Modifications: | ||
| Anealing Duplex for two | ea | $ 25.00 |
| Mod. - 3' Amino Modifier, 100nm scale | ea | $ 28.00 |
| Mod - 3' Phosphorylation, 100 nmole scale | ea | $ 28.00 |
| Mod. - 3' TAMARA, 1.0 umole | ea | $ 145.80 |
| Mod. - 3' TAMARA, 250 nmole scale | ea | $ 100.00 |
| Mod - 3' Thiol Modifier C3, 250 scale | ea | $ 110.00 |
| Mod.-3Tamra, 100nm | ea | $ 85.00 |
| Mod. - 5' Alexa Flour 546, 100nmole scale | ea | $ 295.00 |
| Mod. - 5' Alexa Flour 647, 100nmole scale | ea | $ 345.00 |
| Mod - 5' Amino, 250 scale | ea | $ 33.00 |
| Mod. - 5' Amino Mod C6, 100nm scale | ea | $ 20.00 |
| Mod. - 5' Biotin, 1.0umole scale | ea | $ 100.00 |
| Mod - 5 Biotin, 25/100 nmole scale | ea | $ 38.00 |
| Mod. - 5' Biotin, 250 scale | ea | $ 50.00 |
| Mod - 5 Cy3 100 nmole scale | ea | $ 63.00 |
| Mod - 5 Cy3, 250 nmole scale | ea | $ 90.00 |
| Mod - 5 Cy5 100 nmole scale | ea | $ 63.00 |
| Mod - 5 Cy5, 250 nmole scale | ea | $90.00 |
| Mod. - 5' DigN | ea | $ 103.50 |
| Mod. - 5' FAM, 1.0 umole | ea | $ 145.80 |
| Mod. - 5 FAM, 100 nmole scale | ea | $ 57.00 |
| Mod. - 5' FAM, 250 nmole scale | ea | $ 75.00 |
| Mod - 5 Hex, 100 nmole scale | ea | $ 57.00 |
| Mod. - 5' Hex / 3' Black Hole Quencher 2, 100nm | ea | $ 211.90 |
| Mod - 5' Phosphorylation, 1000nmole | ea | $ 45.00 |
| Mod - 5 Phosphorylation, 25/100 nmole scale | ea | $ 17.00 |
| Mod. - 5' Phosphorylation, 250 nmole scale | ea | $ 25.00 |
| Mod. - 5' Tamara NHS Ester, 1.0umole scale | ea | $ 225.00 |
| Mod. - 5' Tamra, 250 scale | ea | $ 150.00 |
| Mod - 5' Tamra NHS Ester, 100nm scale | ea | $ 125.00 |
| Mod - Black Hole Quencher, 100 nmole | ea | $ 76.35 |
| Mod - Internal Inosine, 100 nmole scale | ea | $ 8.00 |
| Mod - Internal Inosine, 250 nmole scale | ea | $ 10.00 |
| Mod. - JOE 5', 250 scale | ea | $ 150.00 |
| Mod. - Na+ Salt Exchange | ea | $ 45.00 |
| Mod. - ROX 5', 250 scale | ea | $ 150.00 |
| Mod - Thio-ester, 250 nm scale | ea | $ 3.40 |
- Delivery: Custom synthesized oligos are shipped 24 hours from receipt of order by standard overnight delivery. Most modified oligos and those requiring HPLC or PAGE purification are shipped within 5 working days.
- No delivery or shipping fees.
Technical Information
Oligos are delivered lyophilized in individual vials or in 96 or 384 well plates. Each oligo is accompanied by a spec sheet listing your sequence name, sequence, MW, G/C content, Tm, OD, mg and nanomole yield. At no extra charge, every oligo is deprotected and desalted to remove small molecule impurities and is quantitated twice by UV spectrophotometry to provide an accurate measure of yield. Mass yield (OD) varies with length and decreases for shorter oligos. Any synthesis not meeting the yield guarantee is remade. In addition, we statistically sample 100 nmole oligos and perform Gel Electrophoresis. Routinely, every oligo synthesized at the 250 nmole scale or larger and all modified oligos 100 nmole or larger (standard and/or PAGE purified) are individually analyzed by Gel Electrophoresis. MALDI-TOF Mass Spec is run on all modified, purified and plate oligos up to 50 bases in length. IDT can provide you with this documentation for $25 per oligo. Analytical RP-HPLC and/or Capillary Electrophoresis of your oligonucleotide is also available for $25/sample. Additional Purification is recommended for cloning, mutagenesis, assays requiring high sensitivity and all oligos greater than 40 bases in length. Please see our web site or catalog for recommendations on additional purification. We also have an extensive selection of modifications available including dual labeled fluorescent probes for real time PCR, Custom RNA and Antisense Oligos. Our technical support staff of experienced scientists if available for free consultation about your DNA application at (800) 328-2661.
Millipore Sigma
- Internet: http://www.sigmaaldrich.com/
- Technical Support: (800) 325-5832
Ordering Information
Place your order by:
- E-Mail: Following the Millipore Sigma formatting instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.
Pricing Information
- 3 OD Select – $0.14 per base
- 50nm umol scale – $0.20 per base
- 200 nmole scale – $0.50 per base
- 1000 nmole scale – $0.88 per base
Setup fee: No charge (Desalted)
Setup charges:
| Cartridge | 50/200nm | $5.50 |
| Cartridge | 1000nm | $8.80 |
| HPLC | 50/200nm | $29.70 |
| HPLC | 1000nm | $42.90 |
| PAGE | 50/200nm | $29.70 |
| PAGE | 1000nm | $42.90 |
No delivery or shipping fees
Delivery:
| 35 bases | 3rd business day |
| 5’ modifications or 5’ Oligos | within 5 business days |
| HPLC and PAGE purity | within 5 business days |
Cell Center must receive order by 2:30p.m. (FAX or e-mail)
Modifications:
| 5’Fluorescein: | 50/200nm - $29.70 | 1000 nm - $60.50 |
| 5’Phosphorylation: | 50/200nm - $20 | 1000 nm - $40.25 |
| 5’Biotin: | 50/200 nm - $24.20 | 1000 nm - $49.50 |
| Inosine base: | 50/200 nm - $7.70 | 1000 nm - $14.30 |
| Mixed bases: |
Please contact the Stockroom for more information regarding Millipore Sigma oligos.
Technical Information
Millipore Sigma oligos are synthesized using our patented AbacusTM platform which yields a higher coupling efficiency than most competitor platforms. We offer 4 levels of purification: desalt, reverse phase cartridge (RP1), PAGE and reverse phase HPLC. Each and every oligo is physically tested by gel electrophoresis and spectrophotometer analysis. Additionally, each synthesis run is statistically tested using MALDI-ToF mass spectrometry analysis. At Sigma-Genosys, quality is not just a goal – it is a guarantee. In addition to our stringent quality analysis, you will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label that can be used in your records or on an additional vial. All this at no extra charge.
Invitrogen (Life Technologies)
- Internet: http://www.lifetech.com
- Technical Support: (800) 234-3693
Ordering Information
Place your order by:
- Web (*email shoping cart to ccoligo@mail.med.upenn.edu): www.invitrogen.com
- Excel Template for Email orders:
http://tools.thermofisher.com/ downloads/DNA_Tube_template_ USA_final.xls - E-Mail: Following the Invitrogen formating instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.
Pricing Information
- 25 nmole scale (>0.5 OD) – $0.24 per base
- 50 nmole scale (>2 OD) – $0.41 per base
- 200 nmole scale (>10 OD) – $0.90 per base
- 1000 nmole scale (>50 OD) – $1.95 per base
Setup fee: No charge (Desalted).
Setup charges:
| Cartridge | 50/200nm | $25.00 |
| Cartridge | 1000nm | $25.00 |
| HPLC | 200nm | $52.75 |
| HPLC | 50nm | $35.00 |
| HPLC | 1000nm | $77.00 |
| PAGE | 200nm | $64.80 |
| PAGE | 50nm | $45.00 |
| PAGE | 1000nm | $75.00 |
No delivery or shipping fees
Delivery:
| 60 bases, 5’ Phosphorylation, Iosine, Uracil or Cartridge Purity | 3rd business day |
| > 60 bases, 1000 nmole scale, 5’ modifications (except Phosphorylation) or 5’ Oligos | within 5 business days |
| HPLC and PAGE purity | within 6 business days |
Cell Center must receive order by 2:30p.m. (fax or e-mail)
Modifications:
| 5’Phosphorylation: | 25/50nm - $6.70 | 200nm - $9.00 |
| 5’Biotin: | 25/50nm - $35.00 | 200nm - $70.00 |
| Inosine: | 50/200 nm - $8.00 | 1000 nm - $16.00 |
| Mixed bases: | ||
| Uracil: | 50/200 nm - $5 | 1000 nm - $10 |
Technical Information
LTI Custom Primers are synthesized on a highly automated computer controlled parallel array synthesizer using standard phosphoramidite chemistry. Each order is double entered into the system ensuring correct input. Coupling efficiencies are monitored throughout synthesis on each oligonucleotide by trityl analysis. Five levels of purity are currently available: deprotected only; deprotected/desalted by C18 spin-column chromatography; cartridge; HPLC and PAGE. A comprehensive certificate of analysis accompanies each oligonucleotide indicating quantity, molecular weight, extinction coefficient, sequence, and melting temperature.