Oligonucleotide Synthesis
The Stockroom offers oligonucleotide synthesis through three different vendors:
**All orders that have 10 or more sequences must be submitted in excel format. Four columns are required; Sequence name, Sequence, Scale, Purification.**
IDT (Integrated DNA Technologies)
Click here to place an order.
Pricing Information
| Description | Unit | Price |
|---|---|---|
| Base Charges: | ||
| Oligo 25 nmole | per base | $0.38 |
| Oligo 100 nmole | per base | $0.70 |
| Oligo 250 nmole | per base | $1.65 |
| Oligo 1000 nmole | per base | $2.71 |
| Oligo - 10 umole scale | per base | $15.40 |
| Set-Up Charges: | ||
| Setup Charge - HPLC-100 nmole | EA | $46.52 |
| Setup Charge - HPLC-250 nmole | EA | $78.21 |
| Setup Charge - HPLC-1000 nmole | EA | $118.80 |
| Setup Charge - PAGE-100 nmole | EA | $68.31 |
| Setup Charge - PAGE-250 nmole | EA | $97.02 |
| Setup Charge - PAGE-1000 nmole | EA | $120.00 |
| Setup Charge - PAGE (4nm) Ultramer DNA Oligo | EA | $133.65 |
| Setup Charge - Rnase Free HPLC, 100 nmole scale | EA | $84.97 |
| Setup Charge - RNase Free HPLC, 250 -10 nm scale | EA | $101.71 |
| Setup Charge - RNase-Free HPLC purification, 40nm/1um | EA | $157.02 |
| Setup Charge - RNase-Free HPLC, 5um scale | EA | $340.00 |
- Delivery: Custom synthesized oligos are shipped 24 hours from receipt of order by standard overnight delivery. Most modified oligos and those requiring HPLC or PAGE purification are shipped within 5 working days.
- No delivery or shipping fees.
Technical Information
Oligos are delivered lyophilized in individual vials or in 96 or 384 well plates. Each oligo is accompanied by a spec sheet listing your sequence name, sequence, MW, G/C content, Tm, OD, mg and nanomole yield. At no extra charge, every oligo is deprotected and desalted to remove small molecule impurities and is quantitated twice by UV spectrophotometry to provide an accurate measure of yield. Mass yield (OD) varies with length and decreases for shorter oligos. Any synthesis not meeting the yield guarantee is remade. In addition, we statistically sample 100 nmole oligos and perform Gel Electrophoresis. Routinely, every oligo synthesized at the 250 nmole scale or larger and all modified oligos 100 nmole or larger (standard and/or PAGE purified) are individually analyzed by Gel Electrophoresis. MALDI-TOF Mass Spec is run on all modified, purified and plate oligos up to 50 bases in length. IDT can provide you with this documentation for $25 per oligo. Analytical RP-HPLC and/or Capillary Electrophoresis of your oligonucleotide is also available for $25/sample. Additional Purification is recommended for cloning, mutagenesis, assays requiring high sensitivity and all oligos greater than 40 bases in length. Please see our web site or catalog for recommendations on additional purification. We also have an extensive selection of modifications available including dual labeled fluorescent probes for real time PCR, Custom RNA and Antisense Oligos. Our technical support staff of experienced scientists if available for free consultation about your DNA application at (800) 328-2661.
Millipore Sigma
- Internet: http://www.sigmaaldrich.com/
- Technical Support: (800) 325-5832
Ordering Information
Place your order by:
- E-Mail: Following the Millipore Sigma formatting instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.
Pricing Information
| Description | Unit | Price |
|---|---|---|
| Base Charges: | ||
| Oligo - 25nm scale | per base | $ 0.22 |
| Oligo - 50nm scale | per base | $ 0.27 |
| Oligo - 200nm scale | per base | $ 0.52 |
| Oligo - 1000nm scale | per base | $ 0.90 |
| Setup Charges: | ||
| Setup Charge - HPLC 1000nm | EA | $ 43.14 |
| Setup Charge - HPLC 50/200nm | EA | $ 33.29 |
| Setup charge - PAGE 50/200nm scale | EA | $ 29.89 |
| Setup Charge - RP1 Cartridge 1000nm | EA | $ 8.85 |
| Setup charge - RP1 Cartridge 50/200nm scale | EA | $ 5.53 |
- No delivery or shipping fees
- Please contact the Stockroom for more information regarding Millipore Sigma oligos.
Technical Information
Millipore Sigma oligos are synthesized using our patented AbacusTM platform which yields a higher coupling efficiency than most competitor platforms. We offer 4 levels of purification: desalt, reverse phase cartridge (RP1), PAGE and reverse phase HPLC. Each and every oligo is physically tested by gel electrophoresis and spectrophotometer analysis. Additionally, each synthesis run is statistically tested using MALDI-ToF mass spectrometry analysis. At Sigma-Genosys, quality is not just a goal – it is a guarantee. In addition to our stringent quality analysis, you will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label that can be used in your records or on an additional vial. All this at no extra charge.
Invitrogen (Life Technologies)
- Internet: http://www.lifetech.com
- Technical Support: (800) 234-3693
Ordering Information
Place your order by:
- Web (*email shoping cart to ccoligo@mail.med.upenn.edu): www.invitrogen.com
- Excel Template for Email orders:
http://tools.thermofisher.com/ downloads/DNA_Tube_template_ USA_final.xls - E-Mail: Following the Invitrogen formating instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.
Pricing Information
| Description | Unit | Price |
|---|---|---|
| Base Charges: | ||
| 25 nmole scale (>0.5 OD) | per base | $0.32 |
| 50 nmole scale (>2 OD) | per base | $0.64 |
| 200 nmole scale (>10 OD) | per base | $1.14 |
| 1000 nmole scale (>50 OD) | per base | $2.01 |
| Setup Charges: | ||
| Setup charge - Cartridge 50/200nm | ea | $ 29.97 |
| Setup charge - HPLC 50 nmole | ea | $ 38.02 |
| Setup charge - HPLC 200nm | ea | $ 68.55 |
| Setup charge - HPLC 1000nm | ea | $ 79.70 |
| Setup charge - PAGE 50 nmole | ea | $ 58.91 |
| Setup charge - PAGE 200nm | ea | $ 83.41 |
| Setup charge - PAGE 1000nm | ea | $ 90.02 |
- Setup fee: No charge (Desalted)
- No delivery or shipping fees
Technical Information
LTI Custom Primers are synthesized on a highly automated computer controlled parallel array synthesizer using standard phosphoramidite chemistry. Each order is double entered into the system ensuring correct input. Coupling efficiencies are monitored throughout synthesis on each oligonucleotide by trityl analysis. Five levels of purity are currently available: deprotected only; deprotected/desalted by C18 spin-column chromatography; cartridge; HPLC and PAGE. A comprehensive certificate of analysis accompanies each oligonucleotide indicating quantity, molecular weight, extinction coefficient, sequence, and melting temperature.