Oligonucleotide Synthesis

The Stockroom offers oligonucleotide synthesis through three different vendors:

**All orders that have 10 or more sequences must be submitted in excel format. Four columns are required; Sequence name, Sequence, Scale, Purification.**

IDT (Integrated DNA Technologies)

Click here to place an order.

Pricing Information

DescriptionUnitPrice
Base Charges:
Oligo 25 nmoleper base$ 0.24
Oligo 100 nmoleper base$ 0.41
Oligo 250 nmoleper base$ 0.63
Oligo 1000 nmoleper base$ 1.15
Oligo - 10 umole scaleper base$ 16.00
Chimeric DNA baseper base$ 2.48
RNA base, 5 OD scaleper base$ 3.47
Phosphorothioate, 100/250nmoleper base$ 3.33
Phosphorothioate, 1000nmoleper base$ 4.76
Set-Up Charges:
Dual Labeled 5' TET/ 3' BHQ, HPLC, 100nmea$ 189.00
Dual Labeled 6FAM/BHQ, HPLC, 100 nmoleea$ 175.00
Dual Labeled 6FAM/TAMARA, 100 nmoleea$ 175.00
Dual labeled Probe - 6FAM/TAMARA, 250 nmoleea$ 250.00
Dual PAGE & RP-HPLCea$ 85.00
Dual PAGE & RP-HPLCea$ 125.00
Dual PAGE & RP-HPLCea$ 230.00
HPLC-100 nmole set upea$ 40.00
HPLC-1000 nmole set upea$ 95.00
HPLC-250 nmole set upea$ 60.00
IEHPLC-100 nmole set upea$ 40.00
IE-HPLC-1000 nmole set upea$ 95.00
IE-HPLC-250 nmole set upea$ 60.00
PAGE-100 nmole set upea$ 40.00
PAGE-1000 nmole set upea$ 95.00
PAGE-250 nmole set upea$ 60.00
Set Up - RNase Free HPLC, 100 nmoleea$ 65.00
Set Up - RNase Free HPLC, 250 nmoleea$ 91.50
Dyes and Other Modifications:
Anealing Duplex for twoea$ 25.00
Mod. - 3' Amino Modifier, 100nm scaleea$ 28.00
Mod - 3' Phosphorylation, 100 nmole scaleea$ 28.00
Mod. - 3' TAMARA, 1.0 umoleea$ 145.80
Mod. - 3' TAMARA, 250 nmole scaleea$ 100.00
Mod - 3' Thiol Modifier C3, 250 scaleea$ 110.00
Mod.-3Tamra, 100nmea$ 85.00
Mod. - 5' Alexa Flour 546, 100nmole scaleea$ 295.00
Mod. - 5' Alexa Flour 647, 100nmole scaleea$ 345.00
Mod - 5' Amino, 250 scaleea$ 33.00
Mod. - 5' Amino Mod C6, 100nm scaleea$ 20.00
Mod. - 5' Biotin, 1.0umole scaleea$ 100.00
Mod - 5 Biotin, 25/100 nmole scaleea$ 38.00
Mod. - 5' Biotin, 250 scaleea$ 50.00
Mod - 5 Cy3 100 nmole scaleea$ 63.00
Mod - 5 Cy3, 250 nmole scaleea$ 90.00
Mod - 5 Cy5 100 nmole scaleea$ 63.00
Mod - 5 Cy5, 250 nmole scaleea$90.00
Mod. - 5' DigNea$ 103.50
Mod. - 5' FAM, 1.0 umoleea$ 145.80
Mod. - 5 FAM, 100 nmole scaleea$ 57.00
Mod. - 5' FAM, 250 nmole scaleea$ 75.00
Mod - 5 Hex, 100 nmole scaleea$ 57.00
Mod. - 5' Hex / 3' Black Hole Quencher 2, 100nmea$ 211.90
Mod - 5' Phosphorylation, 1000nmoleea$ 45.00
Mod - 5 Phosphorylation, 25/100 nmole scaleea$ 17.00
Mod. - 5' Phosphorylation, 250 nmole scaleea$ 25.00
Mod. - 5' Tamara NHS Ester, 1.0umole scaleea$ 225.00
Mod. - 5' Tamra, 250 scaleea$ 150.00
Mod - 5' Tamra NHS Ester, 100nm scaleea$ 125.00
Mod - Black Hole Quencher, 100 nmoleea$ 76.35
Mod - Internal Inosine, 100 nmole scaleea$ 8.00
Mod - Internal Inosine, 250 nmole scaleea$ 10.00
Mod. - JOE 5', 250 scaleea$ 150.00
Mod. - Na+ Salt Exchangeea$ 45.00
Mod. - ROX 5', 250 scaleea$ 150.00
Mod - Thio-ester, 250 nm scaleea$ 3.40
  • Delivery: Custom synthesized oligos are shipped 24 hours from receipt of order by standard overnight delivery. Most modified oligos and those requiring HPLC or PAGE purification are shipped within 5 working days.
  • No delivery or shipping fees.

Technical Information

Oligos are delivered lyophilized in individual vials or in 96 or 384 well plates. Each oligo is accompanied by a spec sheet listing your sequence name, sequence, MW, G/C content, Tm, OD, mg and nanomole yield. At no extra charge, every oligo is deprotected and desalted to remove small molecule impurities and is quantitated twice by UV spectrophotometry to provide an accurate measure of yield. Mass yield (OD) varies with length and decreases for shorter oligos. Any synthesis not meeting the yield guarantee is remade. In addition, we statistically sample 100 nmole oligos and perform Gel Electrophoresis. Routinely, every oligo synthesized at the 250 nmole scale or larger and all modified oligos 100 nmole or larger (standard and/or PAGE purified) are individually analyzed by Gel Electrophoresis. MALDI-TOF Mass Spec is run on all modified, purified and plate oligos up to 50 bases in length. IDT can provide you with this documentation for $25 per oligo. Analytical RP-HPLC and/or Capillary Electrophoresis of your oligonucleotide is also available for $25/sample. Additional Purification is recommended for cloning, mutagenesis, assays requiring high sensitivity and all oligos greater than 40 bases in length. Please see our web site or catalog for recommendations on additional purification. We also have an extensive selection of modifications available including dual labeled fluorescent probes for real time PCR, Custom RNA and Antisense Oligos. Our technical support staff of experienced scientists if available for free consultation about your DNA application at (800) 328-2661.

Sigma-Genosys

Ordering Information

Place your order by:

  • E-Mail: Following the Sigma-Genosys formating instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.

Pricing Information

  • 3 OD Select – $0.14 per base
  • 50nm umol scale – $0.20 per base
  • 200 nmole scale – $0.50 per base
  • 1000 nmole scale – $0.88 per base

Setup fee: No charge (Desalted)

Setup charges:

Cartridge50/200nm$5.50
Cartridge1000nm$8.80
HPLC50/200nm$29.70
HPLC1000nm$42.90
PAGE50/200nm$29.70
PAGE1000nm$42.90

No delivery or shipping fees

Delivery:

35 bases3rd business day
5’ modifications or 5’ Oligoswithin 5 business days
HPLC and PAGE puritywithin 5 business days

Cell Center must receive order by 2:30p.m. (FAX or e-mail)

Modifications:

5’Fluorescein:50/200nm - $29.701000 nm - $60.50
5’Phosphorylation:50/200nm - $201000 nm - $40.25
5’Biotin:50/200 nm - $24.201000 nm - $49.50
Inosine base:50/200 nm - $7.701000 nm - $14.30
Mixed bases:

Please contact the Stockroom for more information regarding Sigma-Genosys oligos.

Technical Information

Sigma-Genosys oligos are synthesized using our patented AbacusTM platform which yields a higher coupling efficiency than most competitor platforms. We offer 4 levels of purification: desalt, reverse phase cartridge (RP1), PAGE and reverse phase HPLC. Each and every oligo is physically tested by gel electrophoresis and spectrophotometer analysis. Additionally, each synthesis run is statistically tested using MALDI-ToF mass spectrometry analysis. At Sigma-Genosys, quality is not just a goal – it is a guarantee. In addition to our stringent quality analysis, you will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo. You will also receive an additional label that can be used in your records or on an additional vial. All this at no extra charge.

Invitrogen (Life Technologies)

  • Internet: http://www.lifetech.com
  • Technical Support: (800) 234-3693

Ordering Information

Place your order by:

  • Web (*email shoping cart to ccoligo@mail.med.upenn.edu): www.invitrogen.com
  • Excel Template for Email orders:
    http://tools.thermofisher.com/ downloads/DNA_Tube_template_ USA_final.xls
  • E-Mail: Following the Invitrogen formating instructions for sending custom oligo orders via email, send order to ccoligo@mail.med.upenn.edu by 2:30 p.m.

Pricing Information

  • 25 nmole scale (>0.5 OD) – $0.24 per base
  • 50 nmole scale (>2 OD) – $0.41 per base
  • 200 nmole scale (>10 OD) – $0.90 per base
  • 1000 nmole scale (>50 OD) – $1.95 per base

Setup fee: No charge (Desalted).

Setup charges:

Cartridge50/200nm$25.00
Cartridge1000nm$25.00
HPLC200nm$52.75
HPLC50nm$35.00
HPLC1000nm$77.00
PAGE200nm$64.80
PAGE50nm$45.00
PAGE1000nm$75.00

No delivery or shipping fees

Delivery:

60 bases, 5’ Phosphorylation, Iosine, Uracil or Cartridge Purity3rd business day
> 60 bases, 1000 nmole scale, 5’ modifications (except Phosphorylation) or 5’ Oligoswithin 5 business days
HPLC and PAGE puritywithin 6 business days

Cell Center must receive order by 2:30p.m. (fax or e-mail)

Modifications:

5’Phosphorylation:25/50nm - $6.70200nm - $9.00
5’Biotin:25/50nm - $35.00200nm - $70.00
Inosine:50/200 nm - $8.001000 nm - $16.00
Mixed bases:
Uracil:50/200 nm - $51000 nm - $10

Technical Information

LTI Custom Primers are synthesized on a highly automated computer controlled parallel array synthesizer using standard phosphoramidite chemistry. Each order is double entered into the system ensuring correct input. Coupling efficiencies are monitored throughout synthesis on each oligonucleotide by trityl analysis. Five levels of purity are currently available: deprotected only; deprotected/desalted by C18 spin-column chromatography; cartridge; HPLC and PAGE. A comprehensive certificate of analysis accompanies each oligonucleotide indicating quantity, molecular weight, extinction coefficient, sequence, and melting temperature.

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