Pricing

Sanger Sequencing


ScalePenn & CHOPWistar & MonellOff Campus (non-profit)
Per Sample$6.50$7.20$9.00
Full Plate ( 10 / mo)$300$330n/a
Ready to Load (full plate)$120$132$145
Sequencing Enhancer$.70$.70$.70
Bisulphite SequencingContact FacilityContact FacilityContact Facility
Sequencing ProjectContact FacilityContact FacilityContact Facility
Sequence Analysis/AssemblyContact FacilityContact FacilityContact Facility

1 Prescribed amount of DNA and primer are to be put into each well of a 96-well PCR plate, compatible with the ABI PCR machine. Requests are to be submitted on line.

Ion Torrent Sequencing


Chip314 chip314 chip2316 chip316 chip2318 chip318 chip2
Read Length(200b)(400b)(200b)(400b)(200b)(400b)
Sequenceing (pooled libraries submitted)$650$830$840$1,025$1,025$1210
Barcoded library, gDNA or long amplicons, with Pippin Prep size selection, 1-3 Lib. - $100 each; 4 or more - $75 each
Barcoded library, cancer hotspot panel v2, 8 minimum - $130 each, more for fewer samples
Comprehensive cancer panel or custom panel library - Contact us
Volume discount - Contact us

Ion Torrent Sequencing on S5


Chip520520530530540540
Average Read Length200b & 400b200b & 400b200b & 400b200b & 400b200b only200b only
Sequencing                                         (pooled libraries submitted)$1,090$1,090$1,230$1,230$1,400$1,400
Sequencing                                         (pooled libraries submitted)$1,090$1,090$1,230$1,230$1,400$1,400
Barcoded library, gDNA or long amplicons, with Pippin Prep size selection, 1-3 Lib. - $100 each; 4 or more - $75 each
Barcoded library, cancer hotspot panel v2, 8 minimum - $130 each, more for fewer samples
Comprehensive Cancer Panel or Custom Panel Library - Contact us
AmpliSeq Transcriptome and Total RNA-Seq Library - Contact us
AmpliSeq Exome and Whole Exome Library - Contact us
Volume discount - Contact us

Genotyping and Fragment Analysis on ABI 3130XL


ScalePenn & CHOPWistar & MonellOff Campus (non-profit)
96 samples$170$187$206
48 samples$110$121$133
96 samples (RTL)$110$121$133
48 samples (RTL)$70$77$85
Full Service GenotypingContact FacilityContact FacilityContact Facility
LOH AnalysisContact FacilityContact FacilityContact Facility

  1. Customer provides labeled PCR products. Price includes software based genotyping.
  2. Ready to Load

Human Cell Line Authentication


1-2 cell lines$100 each
3-4 cell lines$65 each
5 or more cell lines$50 each

DNA, RNA and Protein Analysis on Agilent 2100 Bioanalyzer


 Penn & CHOPWistar & Monell
Bioanalyzer DNA1000, ≤ 12 samples50Contact Facility
Bioanalyzer DNA High Sensitivity, ≤ 11 samples72Contact Facility
Bioanalyzer RNA nano, ≤ 12 samples80Contact Facility
Bioanalyzer RNA Pico, ≤ 11 samples80Contact Facility

Size Selection by Pippin Prep for NGS Library


 Penn & CHOPWistar & Monell
1-2 Samples4347
3 Samples5257
4-5 Samples7077

DNA/RNA quantitation on Qubit Fluorometer


Penn & CHOPWistar & MonellOff Campus (non-profit)
$3.50/tail$3.90/tailContact Facility

Mouse Genotyping for Transgene Identification


Penn & CHOPWistar & MonellOff Campus (non-profit)
$3.50/tail$3.90/tailContact Facility

Molecular Biology Services


 Penn & CHOPWistar & MonellOff Campus (non-profit)
Plasmid Miniprep on Qiacube4.555.5
Plasmid or phage Midiprep (1-3 samples)465156
Plasmid or phage Midiprep (> 4 samples)3235.539
Plasmid or phage Maxiprep (1-3 samples)5763.270
Plasmid or phage Maxiprep (> 4 samples)45.65156
Plasmid or phage Megaprep (1-3 samples)103114125
Plasmid or phage Megaprep (> 4 samples)8392101
PCR amplification of template (1-3 samples)10.81213.2
PCR amplification of template (> 4 samples)7.88.69.5
PCR product purification (column)13.715.216.7
PCR product purification (enzymatic)22.22.4
PCR product purification (gel)252830.6
PCR product/DNA fragment purification (AmPure beads)99.910.9
Transformation353841.75
Site-directed Mutation200222244
TA Cloning of PCR product606672.6
Sub-cloning160178196

  1. Mutagenic oligos & sequencing not included
  2. PCR amplification, DNA mini prep not included
  3. DNA mini prep andsequence verification not included

Targeting Vector Construction


 Penn & CHOPWistar & MonellOff Campus (non-profit)
Conditional knock-out construct435043504350
Knock-in construct365036503650

Bioinformatics (For Ion Torrent PGM and Proton data only)


Penn & CHOPWistar & PennOff Campus (non-profit)
$100 / hour

Penn Genomics Analysis Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

Top