Pricing
- Sanger Sequencing
- Ion Torrent PGM Sequencing
- Ion Torrent Sequencing on S5
- Genotyping and Fragment Analysis on ABI 3130XL
- Human Cell Line Authentication
- DNA, RNA and Protein Analysis on Agilent 2100 Bioanalyzer
- Size Selection by Pippin Prep for NGS Library
- DNA/RNA Quantitation on Qubit Fluorometer
- Mouse Genotyping for Transgene Identification
- Molecular Biology Services
- Targeting Vector Construction
- Bioinformatics
Sanger Sequencing
| Scale | Penn & CHOP | Wistar & Monell | Off Campus (non-profit) |
|---|---|---|---|
| Per Sample | $6.50 | $7.20 | $9.00 |
| Full Plate ( 10 / mo) | $300 | $330 | n/a |
| Ready to Load (full plate) | $120 | $132 | $145 |
| Sequencing Enhancer | $.70 | $.70 | $.70 |
| Bisulphite Sequencing | Contact Facility | Contact Facility | Contact Facility |
| Sequencing Project | Contact Facility | Contact Facility | Contact Facility |
| Sequence Analysis/Assembly | Contact Facility | Contact Facility | Contact Facility |
1 Prescribed amount of DNA and primer are to be put into each well of a 96-well PCR plate, compatible with the ABI PCR machine. Requests are to be submitted on line.
Ion Torrent Sequencing
| Chip | 314 chip | 314 chip2 | 316 chip | 316 chip2 | 318 chip | 318 chip2 |
|---|---|---|---|---|---|---|
| Read Length | (200b) | (400b) | (200b) | (400b) | (200b) | (400b) |
| Sequenceing (pooled libraries submitted) | $650 | $830 | $840 | $1,025 | $1,025 | $1210 |
| Barcoded library, gDNA or long amplicons, with Pippin Prep size selection, 1-3 Lib. - $100 each; 4 or more - $75 each | ||||||
| Barcoded library, cancer hotspot panel v2, 8 minimum - $130 each, more for fewer samples | ||||||
| Comprehensive cancer panel or custom panel library - Contact us | ||||||
| Volume discount - Contact us |
Ion Torrent Sequencing on S5
| Chip | 520 | 520 | 530 | 530 | 540 | 540 |
|---|---|---|---|---|---|---|
| Average Read Length | 200b & 400b | 200b & 400b | 200b & 400b | 200b & 400b | 200b only | 200b only |
| Sequencing (pooled libraries submitted) | $1,090 | $1,090 | $1,230 | $1,230 | $1,400 | $1,400 |
| Sequencing (pooled libraries submitted) | $1,090 | $1,090 | $1,230 | $1,230 | $1,400 | $1,400 |
| Barcoded library, gDNA or long amplicons, with Pippin Prep size selection, 1-3 Lib. - $100 each; 4 or more - $75 each | ||||||
| Barcoded library, cancer hotspot panel v2, 8 minimum - $130 each, more for fewer samples | ||||||
| Comprehensive Cancer Panel or Custom Panel Library - Contact us | ||||||
| AmpliSeq Transcriptome and Total RNA-Seq Library - Contact us | ||||||
| AmpliSeq Exome and Whole Exome Library - Contact us | ||||||
| Volume discount - Contact us |
Genotyping and Fragment Analysis on ABI 3130XL
| Scale | Penn & CHOP | Wistar & Monell | Off Campus (non-profit) |
|---|---|---|---|
| 96 samples | $170 | $187 | $206 |
| 48 samples | $110 | $121 | $133 |
| 96 samples (RTL) | $110 | $121 | $133 |
| 48 samples (RTL) | $70 | $77 | $85 |
| Full Service Genotyping | Contact Facility | Contact Facility | Contact Facility |
| LOH Analysis | Contact Facility | Contact Facility | Contact Facility |
- Customer provides labeled PCR products. Price includes software based genotyping.
- Ready to Load
Human Cell Line Authentication
| 1-2 cell lines | $100 each |
|---|---|
| 3-4 cell lines | $65 each |
| 5 or more cell lines | $50 each |
DNA, RNA and Protein Analysis on Agilent 2100 Bioanalyzer
| Penn & CHOP | Wistar & Monell | |
|---|---|---|
| Bioanalyzer DNA1000, ≤ 12 samples | 50 | Contact Facility |
| Bioanalyzer DNA High Sensitivity, ≤ 11 samples | 72 | Contact Facility |
| Bioanalyzer RNA nano, ≤ 12 samples | 80 | Contact Facility |
| Bioanalyzer RNA Pico, ≤ 11 samples | 80 | Contact Facility |
Size Selection by Pippin Prep for NGS Library
| Penn & CHOP | Wistar & Monell | |
|---|---|---|
| 1-2 Samples | 43 | 47 |
| 3 Samples | 52 | 57 |
| 4-5 Samples | 70 | 77 |
DNA/RNA quantitation on Qubit Fluorometer
| Penn & CHOP | Wistar & Monell | Off Campus (non-profit) |
|---|---|---|
| $3.50/tail | $3.90/tail | Contact Facility |
Mouse Genotyping for Transgene Identification
| Penn & CHOP | Wistar & Monell | Off Campus (non-profit) |
|---|---|---|
| $3.50/tail | $3.90/tail | Contact Facility |
Molecular Biology Services
| Penn & CHOP | Wistar & Monell | Off Campus (non-profit) | |
|---|---|---|---|
| Plasmid Miniprep on Qiacube | 4.5 | 5 | 5.5 |
| Plasmid or phage Midiprep (1-3 samples) | 46 | 51 | 56 |
| Plasmid or phage Midiprep (> 4 samples) | 32 | 35.5 | 39 |
| Plasmid or phage Maxiprep (1-3 samples) | 57 | 63.2 | 70 |
| Plasmid or phage Maxiprep (> 4 samples) | 45.6 | 51 | 56 |
| Plasmid or phage Megaprep (1-3 samples) | 103 | 114 | 125 |
| Plasmid or phage Megaprep (> 4 samples) | 83 | 92 | 101 |
| PCR amplification of template (1-3 samples) | 10.8 | 12 | 13.2 |
| PCR amplification of template (> 4 samples) | 7.8 | 8.6 | 9.5 |
| PCR product purification (column) | 13.7 | 15.2 | 16.7 |
| PCR product purification (enzymatic) | 2 | 2.2 | 2.4 |
| PCR product purification (gel) | 25 | 28 | 30.6 |
| PCR product/DNA fragment purification (AmPure beads) | 9 | 9.9 | 10.9 |
| Transformation | 35 | 38 | 41.75 |
| Site-directed Mutation | 200 | 222 | 244 |
| TA Cloning of PCR product | 60 | 66 | 72.6 |
| Sub-cloning | 160 | 178 | 196 |
- Mutagenic oligos & sequencing not included
- PCR amplification, DNA mini prep not included
- DNA mini prep andsequence verification not included
Targeting Vector Construction
| Penn & CHOP | Wistar & Monell | Off Campus (non-profit) | |
|---|---|---|---|
| Conditional knock-out construct | 4350 | 4350 | 4350 |
| Knock-in construct | 3650 | 3650 | 3650 |
Bioinformatics (For Ion Torrent PGM and Proton data only)
| Penn & CHOP | Wistar & Penn | Off Campus (non-profit) |
|---|---|---|
| $100 / hour |