At the present time, the TCMF provides the following services on a routine basis:

DNA Injection

Injection of DNA into mouse zygotes (fertilized oocytes) for the production of transgenic mice or embryos.

ES cell Injection

Injection of embryonic stem cells into blastocysts (3.5-day embryo) for the production of chimeric mice.

RNA Injection

Injection of a mix of Cas9 RNA/prot., gRNA and/or ssDNA/dsDNA into mouse zygotes for the production of site-directed genome-edited mice.

Mouse Line Rederivation

Surgical transfer of embryos into pathogen-free recipient females, where they can develop to term.

Embryo Cryopreservation

Controlled freezing of mouse embryos for preservation in liquid nitrogen.

Sperm Cryopreservation

An alternate and less expensive procedure to preserve important mouse lines.

In-Vitro Fertilization

Through this process, a mouse line that undergoes reproductive obstacles can be rescued.

Alternate services are available upon discussion with the TCMF Core for cryopreservation and rederivation of mouse lines and specialized procedures.