Penn Genomics and Sequencing Core

The Penn Genomics and Sequencing Core (PGSC) provides a spectrum of sequencing, genomics and molecular biological services with a team of highly experienced and trained professionals under a Director and Technical Director. The services include consultation on experimental design to performing experiment to downstream data analyses.

We offer Next-Generation Sequencing on multiple platforms:

  • Short Read - Illumina NovaSeq X Plus, NextSeq 2000 and MiSeq
  • Long Read - PacBio Revio and Oxford Nanopore MinION

For more information, go to iLab.

This includes DNA/RNA extraction from different sources, library preparation with quality control, sequencing followed by bioinformatics.

Applications include mRNA-Seq, total RNA-Seq, small RNA-Seq, WGS, targeted sequencing, ChIP-Seq on X Plus; WGS and whole methylome on PacBio etc.

For single-cell genomics we use 10X Genomics Controller; the applications include scRNA-Seq and snRNA-Seq. Soon we are going to introduce multiplexed scRNA-Seq. Multiome including scATAC-Seq are under development.

Bioinformatics support is available for both core and externally generated datasets.

Targeted molecular profiling including genotyping and gene expression is performed using ThermoFisher QS6 Pro and Qiagen digital PCR instruments. Mouse genotyping is done using PCR-based approach.

For more information, go to iLab.

For further information please contact Director, Tapan Ganguly and Technical Director, Erik Toorens.

Charges for Major NGS Services

NovaSeq X Plus

flow cell cycles Million Reads Gbp Lanes Full Service price/Lane Ready to Sequence price (per flow cell, client provides reagents)
X 1.5B 100 1500 150 2 $1,319.56 $442.50
X 1.5B 200 1500 300 2 $1,609.25 $487.50
X 1.5B 300 1500 450 2 $1,725.13 $532.50
X 10B 100 10000 1000 8 $1,228.59 $1,087.50
X 10B 200 10000 2000 8 $1,534.38 $1,287.50
X 10B 300 10000 3000 8 $1,630.94 $1,437.50
X 25B 100 25000 2500 8 $1,914.06 $1,437.50
X 25B 200 25000 5000 8 $2,351.56 $2,087.50
X 25B 300 25000 7500 8 $2,660.94 $2,587.50

NextSeq2000 and MiSeq: Please Contact Us

Illumina Libraries

Library Price per Library
Illumina Stranded mRNA $150
Illumina Stranded Total RNA $194
Single Cell, 10x3' Gene Expression (GEM-X, 20K cells) from $2182
Single Cell, 10x3' Gene Expression (GEM-X, Multiplexed, 5K cells) from $1156
Illumina WGS, PCR-Free Library $120
Twist Capture (Exome, custom panel) $165 plus probe cost
Illumina TSO500, miRNA-seq, 10x ATAC, 10x Multiome See iLab or Contact Us

Pac Bio Revio

Service Price
SMRT Cell Sequencing, 1 SMRT cell $1,700
SMRT Bell 3.0 WGS Library (first) $625
SMRT Bell 3.0 WGS Library (additional) $275
IsoSeq/Kinnex Library (first) for RNA $1,000
IsoSeq/Kinnex Library (additional) for RNA $350

Penn Genomics and Sequencing Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.