Mouse line rederivation

This process involves the surgical transfer of embryos into pathogen-free recipient females, where they can develop to term. Rederivation is the most effective method to rid a colony of pathogenic infections. It is also recommended when genetically altered animals are acquired from outside institutions that do not fall into the category of approved vendors. With the proper procedure, mouse lines can be saved or transferred safely across distant locations.

Due to the diverse environmental conditions among the animal facilities located across campus, the structure of this service will be tailored to fit each and every facility receiving the rederived line. As a consequence, the fees may vary accordingly. Basically, a total of 50 embryos generated from the designated mouse line will be transferred into recipient pathogen-free females that would carry them to term in a housing location assigned by ULAR. The mice born from such transfers should be of sufficient number to rejuvenate or establish the line.

Alternate Services Available from the TCMF Core for Cryopreservation and Re-derivation of Mouse Lines

High efficiency of embryo freezing will depend primarily on the reproductive level of the breeders since low mating frequency and low percentage of fertilization will lead to a prolonged time-consuming process.

Investigator’s Responsibilites

The investigator should contact Jean Richa (215/573-3023) to discuss the plans for the service. These would include the availability and housing location of the line(s) to be rederived, as well as the service fee.