Rotem Karni, PhD

Associate Professor of Genetics

Department: Genetics

Contact Information

One Ucity square, floor 4, room 4018
Philadelphia, 19104
Office: 2158985073
Lab Phone: 2158985072
Email:

Links

Education

  • BsC
    The Hebrew University of Jerusalem, 1997
  • Ph.D. (Biological Chemistry)
    The Hebrew University of Jerusalem, Israel., 2002

Post-Graduate Training

  • Postdoctoral Fellow
    Cold Spring Harbor Laboratory, NY, 2002 - 2007

Selected Publications

  • Lea Cohen-Attali, Yelena Mostinski, Guy Biber, Daniela Kaludjerski, Mor Hanan, Reut Eshel, Alexandra Polyansky, Igor Pery, Aric Orbach, Rotem Karni, Amir Mor : FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN) bioRxiv 2026.03.22.713449; doi: https://doi.org/10.64898/2026.03.22.713449 2026
  • Shlomit Ezer, Shira Yanovsky-Dagan, Amit Granit, Matthew McDougal, Taeyoung Hwang, Israel Antman, Rotem Karni, Wan Hee Yoon, Ann Saada, Adi Inbal, Tamar Harel : Allele-specific antisense oligonucleotide treatment rescues atad3-associated phenotype in zebrafish bioRxiv 2026.05.20.726050; doi: https://doi.org/10.64898/2026.05.20.726050 2026
  • Shivang Khandelwal, Ela Elyada, Reut Japha, Manar Abu Diab, Anurag Prabhu, Zahava Siegfried and Rotem Karni. : Molecular characterization of the heterozygous loss of function mutations in the X-linked PCDH19 gene causing PCDH19-Cluster Epilepsy. bioRxiv 2026.03.16.712128; doi: https://doi.org/10.64898/2026.03.16.712128 2026 Notes: 2026.03.16 (uploaded).
  • Carla Azar-Koussa, Marina Sakran, Efraim Rahamim, Anurag V Prabhu, Samar Salem, Adina Heinberg, Zahava Siegfried, Meital Ben-David-Naim, Eran Zimran, Erez Levanon, Zvika Granot, and Rotem Karni : Targeting the 3’ splice site by a decoy oligonucleotide attenuates U2AF1 splicing activity and inhibits leukemia BioRxiv doi: https://doi.org/10.64898/2026.06.30.735457 2026
  • Prabhu, A.V., Azar-Koussa, C., Siegfried, Z. and Karni R. : Spliced to kill: RNA mis-splicing derived cancer neoantigens. Trends Cancer. 11 : 611-613, 2025
  • Shay Tzaban, Priyanga Appasamy, Elad Zisman, Shiri Klein, Reyut Lewis, Houlin Yu, Akanksha Khorgade, Marc A. Schwartz, Moshe Sade-Feldman, Thomas Eisenhaure, Oren Parnas, Aron Popovtzer, Cyrille Cohen, Eric Shifrut, Aziz M. Al’Khafaji, Rotem karni, Galit Eisenberg, Nir Hacohen, Michal Lotem. : RNA splicing dynamics in CD8 T cells uncovers isoforms that impact T cell-mediated cancer immunotherapy bioRxiv 2025 Notes: 2025.09.07.674706; doi: https://doi.org/10.1101/2025.09.07.674706
  • Yehezkel Sztainberg, Maya David Teitelbaum, Ilana Buchumenski, Hagit Porath, Dror Sharon, Eyal Banin, Rotem Karni, Erez Y. Levanon, Ariel Feiglin. : The prevalent NR2E3 c.932G>A mutation induces aberrant splicing that can be rescued using splice-shifting antisense oligonucleotides. bioRxiv 2024 Notes: 2024.05.01.592034; doi: https://doi.org/10.1101/2024.05.01.592034.
  • Rosenberg-Mogilevsky, A., Siegfried, Z. and Karni R. : Generation of tumor neoantigens by RNA splicing perturbation. Trends Cancer. 11:12-24 11 : 12-24, 2024
  • Yu KM, Trimble DJ, Conner CR, Sheinberg DL, Low GMI, Saluja K, Schmitt KM, Karni RJ. : Oligometastatic Parathyroid Carcinoma Presenting as Neck Pain: A Case Report Ear Nose Throat J 102(19) : 635-639, Oct 2023
  • Amar-Schwartz A, Cohen Y, Elhaj A, Ben-Hur V, Siegfried Z, Karni R, Dor T. : Inhibition of nonsense-mediated mRNA decay may improve stop codon read-through therapy for Duchenne muscular dystrophy Hum Mol Genet 32(15) : 2455-2463, Jul 2023