This process involves the surgical transfer of embryos into pathogen-free recipient females, where they can develop to term. Rederivation is the most effective method for eliminating pathogenic infections from a colony. It is also recommended when genetically altered animals are obtained from outside institutions that are not classified as approved vendors. When performed properly, rederivation allows mouse lines to be preserved and safely transferred across distant locations.
Due to variation in environmental conditions across campus animal facilities, the structure of this service will be tailored to fit the specific needs of each facility receiving the rederived line. Consequently, fees may vary accordingly. In general, a total of 50 embryos from the designated mouse line will be transferred into pathogen-free recipient females, who will carry them to term at a housing location assigned by ULAR. The offspring from these transfers should be sufficient to rejuvenate or establish the line.
Investigator’s Responsibilites
The investigator should contact Kathleen Moosbrugger (kthompso@pennmedicine.upenn.edu – 215-573-3023) to discuss the plans for the service. These would include the availability and housing location of the line(s) to be rederived, as well as the service fee.
